标题NEFL Catalog Number：OM640884

提供者：上海笃玛生物科技有限公司发布时间：2024/9/19 阅读次数：52次 >>进入该公司展台

Product Name	NEFL
Antibody Type	Primary Antibodies
Immunogen	Purified recombinant fragment of human NEFL expressed in E. Coli.

Clonality	Monoclonal
Host Species	Unknow
Tested Applications	WBIHCICCFCMICC/IF
	WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More
Species Reactivity	Human

Alternative Names	NFL NF-L NF68 CMT1F CMT2E More
Molecular Weight(MW)	62kDa

Entrez Gene	4747

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application

Figure 1: Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)
Application

Figure 2: Western blot analysis using NEFL mouse mAb against Hela (1) ａnd Jurkat (2) cell lysate.
Application

Figure 3: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application

Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.
Application

Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEFL mouse mAb with DAB staining.
Application

Figure 4: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) ａnd negative control (red).

Application Notes	WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More

Form	Ascitic fluid containing 0.03% sodium azide
Storage Instructions	4℃; -20℃ for long term storage

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