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Product Name | NEFL |
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Antibody Type | Primary Antibodies |
Immunogen | Purified recombinant fragment of human NEFL expressed in E. Coli. |
Clonality | Monoclonal |
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Host Species | Unknow |
Tested Applications | WBIHCICCFCMICC/IF |
WB:1:250~1:1000 More IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
Species Reactivity | Human |
Alternative Names | More |
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Molecular Weight(MW) | 62kDa |
Entrez Gene | 4747 |
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues using NEFL mouse mAb with DAB staining.
Figure 4: Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 5: Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).
Application Notes | WB:1:250~1:1000 More IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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Form | Purified antibody in PBS with 0.05% sodium azide |
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Storage Instructions | 4℃; -20℃ for long term storage |
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