标题NF-L antibody Catalog Number：OM265296

提供者：上海笃玛生物科技有限公司发布时间：2024/9/19 阅读次数：6次 >>进入该公司展台

Product Name	NF-L antibody
Antibody Type	Primary Antibodies
Product description	Mouse Monoclonal antibody [DA2] to NF-LNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, ａnd are composed predominantly of three major proteins called NF-L, NF-M ａnd NF-H. NF-L is the neurofilament light or low molecular weight polypeptide ａnd runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells ａnd their processes in tissue sections ａnd in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig＇s disease or Alzheimer＇s disease. More
Immunogen	NF-L purified from pig spinal cord

Clonality	Monoclonal
Isotype	IgG1
Clone Number	DA2
Host Species	Mouse
Tested Applications	ICC/IFIHC-FIHC-PWB
	ICC/IF:1:200~1:500 IHC-F:1:50~1:100 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. More
Species Reactivity	HumanMammalian species
Concentration	1 mg/ml
Purification	Affinity purified

Gene Synonyms	NF68 NEFL P07196 CMT2E NFL CMT1F NF-L 4747 162280 NF L More
Target	NF-L
Tissue Specificity	Specifically recognizes the light neurofilament subunit or NF-L (~68 kDa).
Light Chain Type	kappa

Application

Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody (DA2) [ - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) taken with a 40X lens. Nuclei are stained blue.
Application

Western Blot: 68kDa Neurofilament Light Antibody (DA2) [ - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including (lane 4).

Application Notes	ICC/IF:1:200~1:500 IHC-F:1:50~1:100 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. More

Form	Liquid
Storage Instructions	Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot ａnd store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer	Ascitic fluid with 0.1% sodium azide

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