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您的位置:首页 > 技术文献 > 产品说明 > NF-L antibody Catalog Number:OM265292

标题NF-L antibody Catalog Number:OM265292

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/9/20   阅读次数:11次 >>进入该公司展台
 

Product Profile

Product Name NF-L antibody
Antibody Type Primary Antibodies
Product description
Rabbit Polyclonal antibody to NF-LNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
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Immunogen
Native NF-L purified from pig spinal cord

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

ICC/IFIHC-FIHC-PWB

 
For IHC-Fr: Use at a dilution of 1:1,000. For IHC-P: Use at a dilution of 1:1,000. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity

HumanMousePigRatMammalian speciesBovine

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
NF68
NEFL
P07196
CMT2E
NFL
CMT1F
NF-L
4747
162280
NF L
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Target NF-L
Tissue Specificity Recognizes the light neurofilament subunit (~68 kDa).

Application

  • Application

    Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody - Mixed neuron/glia cultures from newborn rat brain stained antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cylasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA

  • Application

    Western Blot: Neurofilament light chain Antibody - Western blot of whole rat cerebellum homogenate stained with , at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rodent NF-L. Human, cow and bovine NF-L run a little slower, at about 70kDa.

Positive Control Rat cerebellum
Application Notes
For IHC-Fr: Use at a dilution of 1:1,000. For IHC-P: Use at a dilution of 1:1,000. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer Whole antiserum containing no preservative
 

关键词:稳定性强  特异性强    灵敏度高  

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