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Product Name | NF-L antibody |
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Antibody Type | Primary Antibodies |
Product description |
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Immunogen | Native NF-L purified from pig spinal cord |
Clonality | Polyclonal |
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Host Species | Rabbit |
Tested Applications | ICC/IFIHC-FIHC-PWB |
For IHC-Fr: Use at a dilution of 1:1,000. For IHC-P: Use at a dilution of 1:1,000. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the end user.: | |
Species Reactivity | HumanMousePigRatMammalian speciesBovine |
Concentration | 1 mg/ml |
Purification | Affinity purified |
Gene Synonyms | More |
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Target | NF-L |
Tissue Specificity | Recognizes the light neurofilament subunit (~68 kDa). |
Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody - Mixed neuron/glia cultures from newborn rat brain stained antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cylasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA
Western Blot: Neurofilament light chain Antibody - Western blot of whole rat cerebellum homogenate stained with , at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rodent NF-L. Human, cow and bovine NF-L run a little slower, at about 70kDa.
Positive Control | Rat cerebellum |
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Application Notes | For IHC-Fr: Use at a dilution of 1:1,000. For IHC-P: Use at a dilution of 1:1,000. For WB: Use at a dilution of 1:5,000. Optimal dilutions/concentrations should be determined by the end user.: |
Form | Liquid |
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Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
Storage Buffer | Whole antiserum containing no preservative |
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