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您的位置:首页 > 技术文献 > 产品说明 > Bmi1 antibody Catalog Number:OM241672

标题Bmi1 antibody Catalog Number:OM241672

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/9/20   阅读次数:39次 >>进入该公司展台
 

Product Profile

Product Name Bmi1 antibody
Antibody Type Primary Antibodies
Product description
Rabbit Polyclonal antibody to Bmi1 (BMI1 polycomb ring finger oncogene)Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
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Immunogen
Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IPWB

 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human

Predicted Reactivity Mouse, Rat, Xenopus laevis, Dog, Cat, Pig, Chicken, Chimpanzee, BovineMouse (96%), Rat (92%), Xenopus laevis (82%), Dog (98%), Cat (99%), Pig (99%), Chicken (94%), Chimpanzee (100%), Bovine (98%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
FLVI2/BMI1
PCGF4
BMI1
BMI1 polycomb ring finger oncogene
RNF51
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Target Bmi1
Molecular Weight(MW) 37 kDa (note)
Cellular Localization Nucleus , Cylasm

Application

  • Application

    Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg THP-1 whole cell lysate/extract 10 % SDS-PAGE Bmi1 antibody dilution: 1:3000

  • Application

    Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Bmi1 protein by 4 μg Bmi1 antibody 5 % SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Positive Control K562 , THP-1
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

关键词:稳定性强  特异性强    灵敏度高  

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