标题Bmi1 antibody Catalog Number：OM241672

提供者：上海笃玛生物科技有限公司发布时间：2024/9/20 阅读次数：37次 >>进入该公司展台

Product Name	Bmi1 antibody
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to Bmi1 (BMI1 polycomb ring finger oncogene)Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling ａnd modification of histones; it mediates monoubiquitination of histone H2A ＇Lys-119＇, rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. More
Immunogen	Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	IPWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	Human
Predicted Reactivity	Mouse, Rat, Xenopus laevis, Dog, Cat, Pig, Chicken, Chimpanzee, BovineMouse (96%), Rat (92%), Xenopus laevis (82%), Dog (98%), Cat (99%), Pig (99%), Chicken (94%), Chimpanzee (100%), Bovine (98%)
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Gene Synonyms	FLVI2/BMI1 PCGF4 BMI1 BMI1 polycomb ring finger oncogene RNF51 More
Target	Bmi1
Molecular Weight(MW)	37 kDa (note)
Cellular Localization	Nucleus , Cylasm

Application

Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg THP-1 whole cell lysate/extract 10 % SDS-PAGE Bmi1 antibody dilution: 1:3000
Application

Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Bmi1 protein by 4 μg Bmi1 antibody 5 % SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Positive Control	K562 , THP-1
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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