标题BMI1 Catalog Number：OM640906

提供者：上海笃玛生物科技有限公司发布时间：2024/9/20 阅读次数：33次 >>进入该公司展台

Antibody Type	Primary Antibodies
Immunogen	Purified recombinant fragment of human BMI1 expressed in E. Coli.

Clonality	Monoclonal
Host Species	Unknow
Tested Applications	WBIHCICCFCMICC/IF
	WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More
Species Reactivity	Human

Alternative Names	PCGF4 RNF51 MGC12685 FLVI2/BMI1 More
Molecular Weight(MW)	37kDa

Entrez Gene	648

Application

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Application

Figure 1: Western blot analysis using BMI1 mAb against human BMI1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)
Application

Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BMI1 mouse mAb with DAB staining.
Application

Figure 2: Western blot analysis using BMI1 mAb against HEK293 (1) ａnd BMI1-hIgGFc transfected HEK293 (2) cell lysate.
Application

Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BMI1 mouse mAb with DAB staining.
Application

Figure 4: Immunofluorescence analysis of Hela cells using BMI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application

Figure 5: Flow cytometric analysis of NIH/3T3 cells using BMI1 mouse mAb (green) ａnd negative control (red).

Application Notes	WB:1:250~1:1000 IHC:1:20~1:100 ICC:1:100~1:200 ICC/IF:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More

Form	Ascitic fluid containing 0.03% sodium azide.
Storage Instructions	4℃; -20℃ for long term storage

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