标题Bmi1 [JJ093-3] Catalog Number：OM637714

提供者：上海笃玛生物科技有限公司发布时间：2024/9/20 阅读次数：35次 >>进入该公司展台

Product Name	Bmi1 [JJ093-3]
Antibody Type	Primary Antibodies
Product description	In Drosophila, Polycomb (Pc-g) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. Mel-18 ａnd Bmi-1, mammalian homologs of Drosophila Pc-g group proteins, are similarly expressed during development ａnd implicated in the regulation of gene expression, axial skeleton development, control of proliferation ａnd survival of haemaoietic cells. Mel-18 directly binds to DNA through a RING-finger motif ａnd preferentially associates with juxtaposed enhancer elements on various genes, including Bcl-2, c-Myc ａnd Hox. Mel-18 is an immediate early response gene within the c-Myc/Cdc25 signaling cascade that exhibits tumor suppressor activity ａnd negatively regulates cell cycle progression by blocking S phase entry. Alternatively, Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc ａnd Pim, subsequently resulting in the progression of lymphomagenesis. More
Immunogen	recombinant protein

Alternative Names	B lymphoma Mo MLV insertion region (mouse) antibody B lymphoma Mo MLV insertion region 1 homolog antibody Bmi 1 antibody BMI1 antibody BMI1 polycomb ring finger oncogene antibody BMI1_HUMAN antibody Flvi 2/bmi 1 antibody FLVI2/BMI1 antibody MGC12685 antibody Murine leukemia viral (bmi 1) oncogene homolog antibody Oncogene BMI 1 antibody PCGF 4 antibody PCGF4 antibody Polycomb complex protein BMI 1 antibody Polycomb complex protein BMI-1 antibody Polycomb group protein Bmi1 antibody Polycomb group ring finger 4 antibody Polycomb group RING finger protein 4 antibody RING finger protein 51 antibody RNF 51 antibody RNF51 antibody More
Molecular Weight(MW)	42 kDa
Cellular Localization	Nucleus, Cylasm.

SwissProt ID	P35226 307151

Application

Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: PC-12
Application

Fig2: ICC staining Bmi1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig3: ICC staining Bmi1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig4: ICC staining Bmi1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Application

Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Application

Fig7: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Application

Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

Positive Control	A549, SW480, Hela, K562, PC-12, rat smooth muscle tissue, human tonsil tissue, human lung cancer tissue, human breast carcinoma tissue.
Application Notes	WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

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