标题Bmi1 [A11-C11] Catalog Number：OM626928

提供者：上海笃玛生物科技有限公司发布时间：2024/9/20 阅读次数：2次 >>进入该公司展台

Product Name	Bmi1 [A11-C11]
Antibody Type	Primary Antibodies
Product description	In Drosophila, Polycomb (Pc-g) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. Mel-18 ａnd Bmi-1, mammalian homologs of Drosophila Pc-g group proteins, are similarly expressed during development ａnd implicated in the regulation of gene expression, axial skeleton development, control of proliferation ａnd survival of haemaoietic cells. Mel-18 directly binds to DNA through a RING-finger motif ａnd preferentially associates with juxtaposed enhancer elements on various genes, including Bcl-2, c-Myc ａnd Hox. Mel-18 is an immediate early response gene within the c-Myc/Cdc25 signaling cascade that exhibits tumor suppressor activity ａnd negatively regulates cell cycle progression by blocking S phase entry. Alternatively, Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc ａnd Pim, subsequently resulting in the progression of lymphomagenesis. More
Immunogen	Recombinant protein

Alternative Names	B lymphoma Mo MLV insertion region (mouse) antibody B lymphoma Mo MLV insertion region 1 homolog antibody Bmi 1 antibody BMI1 antibody BMI1 polycomb ring finger oncogene antibody BMI1_HUMAN antibody Flvi 2/bmi 1 antibody FLVI2/BMI1 antibody MGC12685 antibody Murine leukemia viral (bmi 1) oncogene homolog antibody Oncogene BMI 1 antibody PCGF 4 antibody PCGF4 antibody Polycomb complex protein BMI 1 antibody Polycomb complex protein BMI-1 antibody Polycomb group protein Bmi1 antibody Polycomb group ring finger 4 antibody Polycomb group RING finger protein 4 antibody RING finger protein 51 antibody RNF 51 antibody RNF51 antibody More
Molecular Weight(MW)	42 kDa
Cellular Localization	Cylasm. Nucleus.

SwissProt ID	P35226 P25916

Application

Fig1: Western blot analysis of Bmi1 on Hela (1) ａnd NIH-3T3 (2) using anti-Bmi1 antibody at 1/500 dilution.
Application

Fig2: ICC staining Bmi1 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Application

Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

Positive Control	Hela, NIH-3T3, SH-SY-5Y, human colon cancer tissue, mouse spleen tissue.
Application Notes	WB:1:500-1:1,000 ICC:1:200 IHC:1:200-1:400

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

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