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您的位置:首页 > 技术文献 > 产品说明 > alpha Tubulin antibody (ascites) Catalog Number:OM239218

标题alpha Tubulin antibody (ascites) Catalog Number:OM239218

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/9/23   阅读次数:43次 >>进入该公司展台
 

Product Profile

Product Name alpha Tubulin antibody (ascites)
Antibody Type Primary Antibodies
Product description
Mouse monoclonal antibody [DM1A] to alpha TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.GT211302 may be used to study the intracellular organization and distribution of tubulin along the static and dynamic aspects of the cytoskeleton. The product can be used for the immunocytochemical localization of the alpha chain of tubulin by means of indirect immunofluorescent labeling of cultured cells, formalin fixed or frozen tissues or for specific identification of the alpha-tubulin band in immunoblotting or immunoprecipitation techniques.
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Immunogen
Purified chick brain tubulin.

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number DM1A
Host Species Mouse
Tested Applications

ICC/IFIHC-PIPWBIB

 
IF: Use at a dilution of 1/500, this is determined by indirect immunofluorescent labeling of cultured chicken fibroblasts. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user.:
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Species Reactivity

HumanMouseAmphibiansFishFungiHamsterBovine

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
TUBA1
P68366
alpha Tubulin
191110
H2ALPHA
H2-ALPHA
7277
FLJ30169
TUBA1B
TUBA2
TUBA3D
TUBA8
H2 ALPHA
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Target alpha Tubulin
Cellular Localization Major constituent of microtubules.

Application

  • Application

    Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-α-Tubulin, clone no. DM1A with Anti-Mouse-Alexa 488 (green) and DAPI (blue).

  • Application

    Confocal image of the mouth of the Cryptocaryon irritans, a parasite of marine fishes, (theront stage) stained with Monoclonal Anti-α-Tubulin, clone no. DM 1A and Anti-Mouse IgG-lissamine-rhodamine conjugate. Note the large bundles of microtubules which form a well-developed cyhyrigeal basket.

  • Application

    IHC-P analysis of human heart sections using alpha Tubulin antibody [DM1A] at a dilution of 1:500.

  • Application

    WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at a dilution of 1:500.

  • Application

    Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Monoclonal Anti-α-Tubulin . The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lane A: Antibody dilution 1:1,000 Lane B: Negative control (only secondary antibody)

  • Application

    Human skin fibroblasts stained with Monoclonal Anti-α-Tubulin, clone No. DM1A .

Positive Control Chicken fibroblasts
Application Notes
IF: Use at a dilution of 1/500, this is determined by indirect immunofluorescent labeling of cultured chicken fibroblasts. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user.:
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Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer Raw Ascites containing 15mM sodium azide

关键词:稳定性强  特异性强    灵敏度高  

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