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| Product Name | PPP1A (PPP1CA) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB~~1:100~500 IHC~~1:50~100: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Gene Synonyms | |
|---|---|
| Alternative Names | |
| Molecular Weight(MW) | 37512 Da |
| Function | Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, ａnd participates in the regulation of glycogen metabolism, muscle contractility ａnd protein synthesis. Involved in regulation of ionic conductances ａnd long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure ａnd cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity ａnd centrosome number ａnd splitting, both in the presence ａnd absence of radiation-induced DNA damage. Regulator of neural tube ａnd optic fissure closure, ａnd enteric neural crest cell (ENCCs) migration during development More |
| Tissue Specificity | This PPP1A (PPP1CA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human PPP1CA. |
| Cellular Localization | Cylasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Primarily nuclear ａnd largely excluded from the nucleolus. Highly mobile in cells ａnd can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles |
| Entrez Gene | 5499 |
|---|---|
| Nucleotide Accession | NP_002699 |
Western blot analysis of anti-PPP1CA Pab in mouse heart tissue lysate (35ug/lane). PPP1CA(arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed ａnd paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
| Application Notes | WB~~1:100~500 IHC~~1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS. |
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