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您的位置:首页 > 技术文献 > 产品说明 > PPP1A antibody Catalog Number:OM269376

标题PPP1A antibody Catalog Number:OM269376

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/9/24   阅读次数:30次 >>进入该公司展台
 

Product Profile

Product Name PPP1A antibody
Antibody Type Primary Antibodies
Product description
Rabbit Polyclonal antibody to PPP1A (protein phosphatase 1, catalytic subunit, alpha isozyme)The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Immunogen
Recombinant protein encompassing a sequence within the center region of human PPP1A. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIHC-PWB

 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

HumanRat

Predicted Reactivity Mouse, Xenopus laevis, Dog, Pig, Rabbit, BovineMouse (99%), Xenopus laevis (97%), Dog (100%), Pig (100%), Rabbit (97%), Bovine (100%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
MGC15877 antibody
MGC1674 antibody
PP-1A antibody
PP1alpha antibody
PPP1A antibody
PPP1CA antibody
serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit antibody
"protein phosphatase 1
catalytic subunit
alpha isoform antibody"
serine
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Molecular Weight(MW) 38 kDa (note) (2)
Cellular Localization Cylasm

Application

  • Application

    WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    PPP1A antibody detects PPP1A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.

  • Application

    Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A antibody at 1:100 dilution.

Positive Control 293T , rat brain
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

关键词:稳定性强  特异性强    灵敏度高  

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