标题NQO1 Antibody Catalog Number：OM286504

提供者：上海笃玛生物科技有限公司发布时间：2024/9/24 阅读次数：28次 >>进入该公司展台

Product Name	NQO1 Antibody
Antibody Type	Primary Antibodies
Product description	NQO1 is a member of the NAD(P)H dehydrogenase(qui) family ａnd a cylasmic 2-electron reductase. This FAD-binding protein forms homodimers ａnd reduces quis to hydroquis. This protein＇s enzymatic activity prevents the one electron reduction of quis that results in the production of radical species. Altered expression of the protein has been seen in many tumors ａnd is also associated with Alzheimer＇s disease (AD).1) Hubackova,M., Vaclavikova,R. Pharmacogenet. Genomics 19 (7), 505-512 (2009) Canova,C., Hashibe,M. Cancer Res. 69 (7), 2956-2965 (2009) Li,Y. ａnd Jaiswal,A.K. J. Biol. Chem. 267 (21), 15097-15104 (1992) More
Immunogen	This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the Central region of human NQO1.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	FACSIFWB
	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1 mg/ml

Gene Symbol	NQO1
Alternative Names	NAD(P)H dehydrogenase [qui] 1 Azoreductase DT-diaphorase DTD Menadione reductase NAD(P)H:qui oxidoreductase 1 Phylloqui reductase Qui reductase 1 QR1 NQO1 DIA4 NMOR1 More
Molecular Weight(MW)	31 kDa

Entrez Gene	1728
Protein Accession	P15559

Application

Confocal immunofluorescent analysis of NQO1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application

Western blot analysis of NQO1 antibody in NCI-H460 cell line lysates (35ug/lane)
Application

Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

Form	Liquid
Storage Instructions	Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

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