标题NQO1 [JF440-1] Catalog Number：OM628237

提供者：上海笃玛生物科技有限公司发布时间：2024/9/24 阅读次数：39次 >>进入该公司展台

Product Name	NQO1 [JF440-1]
Antibody Type	Primary Antibodies
Product description	NAD(P)H:qui oxidoreductase 1 (NQO1) ａnd NRH:qui oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quis ａnd their derivatives to hydroquis, using either NADH or NADPH as the electron donor. This protects cells against qui-induced oxidative stress, cytotoxicity, ａnd mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins ａnd activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals ａnd radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression ａnd coordinated induction of NQO1. ARE activation by tert-butylhydroqui is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B ａnd Jun-D bind to the ARE ａnd regulate expression ａnd induction of NQO1 gene. Maf-Maf homodimers ａnd possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE ａnd do not repress NQO1 transcription. More
Immunogen	recombinant protein

Alternative Names	Azoreductase antibody Cytochrome b 5 reductase antibody DHQU antibody DIA 4 antibody DIA4 antibody Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody Diaphorase (NADH/NADPH) antibody Diaphorase 4 antibody Dioxin inducible 1 antibody DT diaphorase antibody DT-diaphorase antibody DTD antibody Menadione reductase antibody NAD(P)H dehydrogenase [qui] 1 antibody NAD(P)H dehydrogenase qui 1 antibody NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody NAD(P)H:menadione oxidoreductase 1 antibody NAD(P)H:Qui acceptor oxidoreductase type 1 antibody NAD(P)H:qui oxidoreductase 1 antibody NAD(P)H:qui oxireductase antibody NMOR 1 antibody NMOR I antibody NMOR1 antibody NMORI antibody NQO 1 antibody NQO1 antibody NQO1_HUMAN antibody Phylloqui reductase antibody QR 1 antibody QR1 antibody Qui reductase 1 antibody More
Molecular Weight(MW)	31/27 kDa
Cellular Localization	Cylasm.

SwissProt ID	P15559 Q64669 P05982

Application

Fig1: Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Mouse kidney Lane 3: Raji
Application

Fig2: Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Positive Control	Raji, SH-SY-5Y, mouse kidney tissue.
Application Notes	WB:1:1,000-1:5,000 FC:1:50-1:100 ICC:1:50-1:200 IP:1:50 More

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

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