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| Product Name | MBP |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | KLH conjugated synthetic peptide derived from Gpig MBP 69-85/167 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/test: | |
| Species Reactivity | HumanMouseRatPigGuinea Pig |
| Concentration | 1 mg/mL. |
| Alternative Names | |
|---|---|
| Molecular Weight(MW) | 33 KDa |
| Cellular Localization | Myelin membrane; Peripheral membrane protein; Cylasmic side. Note=Cylasmic side of myelin. |
Fig1: Sample: Brain (Mouse) Lysate at 30 ug Primary: Anti- MBP (ER1912-82) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD
endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MBP) Polyclonal Antibody, Unconjugated (ER1912-82) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Blank control: A549. Primary Antibody (green line): Rabbit Anti-MBP antibody (ER1912-82) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig4: Blank control:A549. Primary Antibody (green line): Rabbit Anti-MBP antibody (ER1912-82) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution:1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
| Positive Control | MBP isoforms are found in both the central ａnd the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen ａnd spinal cord, as well as in cell lines derived from the immune system. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol. |
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