标题PHB Antibody (Center) Catalog Number：OM217034

提供者：上海笃玛生物科技有限公司发布时间：2024/9/26 阅读次数：11次 >>进入该公司展台

Product Name	PHB Antibody (Center)
Antibody Type	Primary Antibodies

Alternative Names	PHB Prohibitin
Molecular Weight(MW)	29804 Da
Function	Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity ａnd in aging
Tissue Specificity	Widely expressed in different tissues.
Cellular Localization	Mitochondrion inner membrane (By similarity)

Entrez Gene	5245

Application

Formalin-fixed ａnd paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application

Western blot analysis of PHB Antibody (Center) in Hela cell line lysates (35ug/lane). PHB (arrow) was detected using the purified Pab.
Application

Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.
Application

Confocal immunofluorescent analysis of PHB Antibody (Center)(Cat#AP2710c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application

PHB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes	IHC~~1:10~50 WB~~1:50~100 IF~~1:10~50 FC~~1:10~50:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

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