标题PHB Antibody (C-term E194) Catalog Number：OM217032

提供者：上海笃玛生物科技有限公司发布时间：2024/9/26 阅读次数：10次 >>进入该公司展台

Product Name	PHB Antibody (C-term E194)
Antibody Type	Primary Antibodies

Alternative Names	PHB Prohibitin
Molecular Weight(MW)	29804 Da
Function	Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity ａnd in aging
Tissue Specificity	Widely expressed in different tissues.
Cellular Localization	Mitochondrion inner membrane (By similarity)

Entrez Gene	5245

Application

Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB gene (Lane 2) (Origene Technologies).
Application

Western blot analysis of anti-PHB Antibody (C-term) in Jurkat cell line lysates (35ug/lane).PHB (arrow) was detected using the purified Pab.
Application

Formalin-fixed ａnd paraffin-embedded human breast carcinoma reacted with PHB Antibody (C-term E194), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application

Flow cytometric analysis of NCI-H292 cells using PHB Antibody (C-term E194)(bottom histogram) compared to a negative control cell ( histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes	WB~~1:50~100 IHC~~1:50~100 FC~~1:10~50:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

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