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您的位置:首页 > 技术文献 > 产品说明 > Prohibitin Antibody Catalog Number:OM288898

标题Prohibitin Antibody Catalog Number:OM288898

   

提供者:上海笃玛生物科技有限公司    发布时间:2024/9/26   阅读次数:31次 >>进入该公司展台
 

Product Profile

Product Name Prohibitin Antibody
Antibody Type Primary Antibodies
Product description
Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.1) Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008) Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008) White,J.J., Genomics 11 (1), 228-230 (1991)
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Immunogen
This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1).

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIHC-PWB

 
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity

Human

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol PHB
Alternative Names
Prohibitin
PHB
Molecular Weight(MW) 30 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine

Database Links

Entrez Gene 5245
Protein Accession P35232

Application

  • Application

    Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow).

  • Application

    Formalin-fixed and paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell ( histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

关键词:稳定性强  特异性强    灵敏度高  

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