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Product Name | Prohibitin Antibody |
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Antibody Type | Primary Antibodies |
Product description |
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Immunogen | This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1). |
Clonality | Polyclonal |
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Isotype | Ig |
Host Species | Rabbit |
Tested Applications | FACSIHC-PWB |
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: | |
Species Reactivity | Human |
Concentration | 1 mg/ml |
Purification | Affinity purified |
Gene Symbol | PHB |
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Alternative Names | |
Molecular Weight(MW) | 30 kDa |
Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Entrez Gene | 5245 |
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Protein Accession | P35232 |
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow).
Formalin-fixed and paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell ( histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
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Form | Liquid |
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Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
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