标题Prohibitin antibody Catalog Number：OM270566

提供者：上海笃玛生物科技有限公司发布时间：2024/9/26 阅读次数：22次 >>进入该公司展台

Product Name	Prohibitin antibody
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to Prohibitin (prohibitin)Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation ａnd may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3＇ untranslated region. The longer transcript is present at higher levels in proliferating tissues ａnd cells, suggesting that this longer 3＇ untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq] More
Immunogen	Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	HumanMouseRat
Predicted Reactivity	Zebrafish, Xenopus laevis, Dog, Cat, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (94%), Xenopus laevis (89%), Dog (100%), Cat (100%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)
Concentration	1 mg/ml
Purification	Affinity purified

Gene Synonyms	PHB1 antibody PHB antibody prohibitin antibody
Molecular Weight(MW)	30 kDa (note) (5)
Cellular Localization	Mitochondrion inner membrane

Application

Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Application

Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000
Application

Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000
Application

Prohibitin antibody detects Prohibitin protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.
Application

Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.
Application

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Prohibitin antibody at 1:500 dilution.
Application

Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE diluted at 1:1000

Positive Control	IMR32 , U87-MG , Neuro2A , GL261 , C8D30 , PC-12 , Rat2
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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