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| Product Name | Prohibitin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. : | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Cat, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (93%), Xenopus laevis (92%), Dog (100%), Cat (100%), Chicken (95%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (3) |
| Cellular Localization | Mitochondrion inner membrane |
Non-transfected (–) ａnd transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, ａnd the membrane was blotted with Prohibitin antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Prohibitin antibody detects Prohibitin protein at mitochondria in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Prohibitin antibody diluted at 1:500.
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, ａnd the membrane was blotted with Prohibitin antibody diluted at 1:1000.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Prohibitin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Sample (30 µg of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Prohibitin antibody detects PHB protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE Prohibitin antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Prohibitin antibody at 1:500 dilution.
Immunoprecipitation of Prohibitin protein from 293T whole cell extracts using 5 μg of Prohibitin antibody . Western blot analysis was performed using Prohibitin antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Prohibitin antibody detects Prohibitin protein at cylasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Prohibitin antibody diluted at 1:500.
Prohibitin antibody detects Prohibitin protein at cylasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Prohibitin antibody diluted at 1:500.
Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.
| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
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