标题MCM2 antibody , N-term Catalog Number：OM262649

提供者：上海笃玛生物科技有限公司发布时间：2024/9/27 阅读次数：23次 >>进入该公司展台

Product Name	MCM2 antibody , N-term
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to MCM2 (minichromosome maintenance complex component 2)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) ａnd may be involved in the formation of replication forks ａnd in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, ａnd 7, ａnd has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, ａnd thus regulated by, protein kinases CDC2 ａnd CDC7. [provided by RefSeq] More
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIPWB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	Human
Predicted Reactivity	Mouse, RatMouse (88%), Rat (88%)
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Gene Synonyms	BM28 antibody CCNL1 antibody CDCL1 antibody D3S3194 antibody KIAA0030 antibody MGC10606 antibody MITOTIN antibody cdc19 antibody MCM2 antibody cell devision cycle-like 1 antibody DNA replication licensing factor MCM2 antibody nuclear protein BM2 More
Molecular Weight(MW)	102 kDa (note)

Application

MCM2 antibody [N1N2], N-term immunoprecipitates MCM2 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 40 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM2 protein by 4 μg MCM2 antibody [N1N2], N-term 7.5 % SDS-PAGE The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application

MCM2 antibody [N1N2], N-term detects MCM2 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Application

Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000

Positive Control	A549 , HeLa , HepG2 , HCT116
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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