标题MCM7 antibody , Internal Catalog Number：OM262674

提供者：上海笃玛生物科技有限公司发布时间：2024/9/27 阅读次数：9次 >>进入该公司展台

Product Name	MCM7 antibody , Internal
Antibody Type	Primary Antibodies
Product description	Rabbit polyclonal antibody to MCM7 (minichromosome maintenance complex component 7)The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) ａnd may be involved in the formation of replication forks ａnd in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein ａnd MCM2, 4 ａnd 6 proteins possesses DNA helicase activity, ａnd may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, ａnd may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq] More
Immunogen	Recombinant protein encompassing a sequence within the center region of human MCM7. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ICC/IFIHC-PIPWBChIP
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	Human
Predicted Reactivity	Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (92%), Rat (93%), Zebrafish (85%), Xenopus laevis (87%), Bovine (98%)
Concentration	1 mg/ml
Purification	Affinity purified

Gene Synonyms	CDC47 antibody MCM2 antibody P1.1-MCM3 antibody P1CDC47 antibody P85MCM antibody PNAS146 antibody MCM7 antibody homolog of S. cerevisiae Cdc47 antibody minichromosome maintenance deficient 7 antibody CDC47 homolog antibody DNA replication licensi More
Target	MCM7
Molecular Weight(MW)	81 kDa (note) (3)
Cellular Localization	Nucleus

Application

MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal 7.5 % SDS-PAGE The immunoprecipitated MCM7 protein was detected by MCM7 antibody [N2C2], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application

Cross-linked ChIP was performed with HeLa chromatin extract ａnd 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
Application

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM7 antibody at 1:200 dilution.
Application

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7 antibody at 1:100 dilution.
Application

Sample (30 µg of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control	293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

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