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| Product Name | Itk Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB~~1:100~500: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
| Gene Synonyms | |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 72292 Da |
| Function | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function ａnd differentiation of conventional T-cells ａnd nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK Phosphorylation leads to ITK auhosphorylation ａnd full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase ａnd subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cylasm ａnd the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein ａnd LCP2. Then, a large number of signaling molecules such as VAV1 are recruited ａnd ultimately lead to lymphokine production, T-cell proliferation ａnd differentiation More |
| Tissue Specificity | Is detected in the thymus, lymph node ａnd very faintly in the spleen, but is not detected in the liver, lung, kidney, heart, brain, intestine or testis. Expressed in T- lymphocytes ａnd mast cells. It may also be expressed in natural killer cells |
| Cellular Localization | Cylasm. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation (By similarity) |
| Entrez Gene | 16428 |
|---|---|
| Nucleotide Accession | NP_034713.2 |
Mouse Itk Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Itk antibody detected the Itk protein (arrow).
Mouse Itk Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Itk antibody detected the Itk protein (arrow).
| Application Notes | WB~~1:100~500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
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