标题HLA-G Antibody Catalog Number：OM281056

提供者：上海笃玛生物科技有限公司发布时间：2024/9/30 阅读次数：66次 >>进入该公司展台

Product Name	HLA-G Antibody
Antibody Type	Primary Antibodies
Product description	HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain ａnd a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa ａnd its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 ａnd 3 encode the alpha1 ａnd alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, ａnd exon 6 encodes the cylasmic tail.1) Walpole,N.G., et.al., J. Mol. Biol. 397 (2), 467-480 (2010) More
Immunogen	This HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	FACSIHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1 mg/ml
Purification	Affinity purified

Gene Symbol	HLA-G
Alternative Names	HLA class I histocompatibility antigen alpha chain G HLA G antigen MHC class I antigen G HLA-G HLA-60 HLAG More
Molecular Weight(MW)	38 kDa

Entrez Gene	3135
Protein Accession	P17693

Application

Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Formalin-fixed ａnd paraffin-embedded human lung carcinoma reacted with HLA-G Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Western blot analysis of HLA-G using rabbit polyclonal HLA-G Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
Application

Western blot analysis of HLA-G Antibody in NCI-H460 cell line lysates (35ug/lane)
Application

Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody ) at 1:1000 at each lane.
Application

Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:

Form	Liquid
Storage Instructions	Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

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