标题Rabbit Anti- CD133 Antigen, FITC conjugated

From：Santa Cruz
Catalog Number : RsF-0338R 

Quantity size : 0.2ml （2mg/ml, Buffer = 0.01M PBS, pH 7.4 with 10 mg/mL BSA ａnd 0.1% Sodium azide. or Lyophilized. Reconstitute with 0.2ml sterile distilled water. ）

Background: Prominin is a novel plasma membrane protein with an N-terminal extracellular domain, five transmembrane segments flanking two short cytoplasmic loops ａnd two large glycosylated extracellular domains, ａnd a cytoplasmic C-terminal domain. DNA sequences from Caenorhabditis elegans predict the existence of a protein with the same features, suggesting that prominin is conserved between vertebrates ａnd invertebrates. Prominin is found not only in the neuroepithelium but also in various other epithelia of the mouse embryo. In the adult mouse, prominin has been detected in the brain ependymal layer, ａnd in kidney tubules. In these epithelia, prominin is specific to the apical surface, where it is selectively associated with microvilli ａnd microvilli-related structures. Remarkably, upon expression in CHO cells, prominin is preferentially localized to plasma membrane protrusions such as filopodia, lamellipodia, ａnd microspikes. These observations imply that prominin contains information to be targeted to, and/or retained in, plasma membrane protrusions rather than the planar cell surface. Moreover, results show that the mechanisms underlying targeting of membrane proteins to microvilli of epithelial cells ａnd to plasma membrane protrusions of non-epithelial cells are highly related.

Specificity : · anti- CD133 antigen is a rabbit monoclonal antibody, FITC conjugated

           · specific for CD133 antigen of human, mouse ａnd rat  

           · epitope mapping near the amino terminus of CD133 antigen of human, mouse ａnd rat origin  

· Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG    

Application : · FCM: 1:20-100

            · Immunohistochemistry : 1:20-1:200

            · Immunocytochemistry: 1:20-1:200

· Excitation spectrum: 490-495nm

· Emission spectrum: 520-530nm

· Optimal working dilutions must be determined by the end user.   
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.